Inverted Microscope: A Great Tool For Studying Living Cells
When you hear the term inverted microscope, you probably think of observing samples from under a microscope. You probably get an image similar to a car mechanic fixing something under a vehicle. It is a funny image but this is not exactly the case when it comes to these kinds of microscopes. The only inverted parts of these tools are the source of light and the objectives that enable you to see the sample plane. Besides, a microscope that places the observer under the equipment will be ill-designed. Just imagine the risks of the specimen toppling and falling onto the observer.
What Does an Inverted Microscope Look Like?
An inverted microscope has the light source and condenser on the top. They point down to the stage where the specimen is placed. The objectives and turret are under the stage. They point up to the bottom of the plane. Just like upright microscopes, the specimen is placed on top of the stage. The binoculars are not pointing up, as one would imagine, but pointing down.
How Does an Inverted Microscope Help in Living Cells Studies
Results of living cells studies should be derived from observations of live cells. This can be done with a typical upright light microscope. The method involves taking a small sample, placing it on glass slide, covering it with a slip, and keeping it moist with water. This is effective and has been used countless of times. However, there are some pitfalls to this method. First of all, by taking a sample from the culture, you are most likely altering the natural processes of the cells. Moreover, the cover slip will put pressure on the specimen, thus altering its processes, too. The water that you use to replenish the sample can also affect its life. Obviously, using an upright light microscope is not ideal for a prolonged observation of living cells.
But if you utilize an inverted microscope, the chances of observing them for a long period of time increases. This is possible for various reasons. One is that you will not have to remove a sample to be placed in a glass slide. The design of the microscope allows for bigger objects to be placed on the sample plane. For instance, if your sample is in a petri dish, you can place it on top of the stage. This way, there will be no pressure on your specimen and the environment will not be altered with constant water replenishment.
Since living cells constantly change from one form to another, being able to observe them for a longer period of time without killing them is essential if you are to study them. Sure these microscopes are a little bit expensive but if living cells study is your field, it is wise to invest in one. The results of your studies will be a whole lot better and you will not regret ever buying it. There are also aspects where the microscope can still be improved but you cannot deny the fact that in most cases, using this microscope is better than using a light microscope or an electron microscope.
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